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The antibody 3RF1pur was purified with Protein-G from in vitro produced monoclonal antibody 3RF1 directed against Na+-Ca+ exchanger protein (see product description 3RF1 for further details concerning immunoblot and immunohistochemistry). The antibody concentration is of 0.18 mg/ml in 0.1M phosphate buffered saline, pH 7.3. 

Immunohistochemistry: we suggest using a dilution of 1:10’000 (0.018 ug/ml) for immunostaining with the avidin-biotin method on cryostat or paraffin-sections of paraformaldehyde (4%) or formalin-fixed tissue. 

We recommend that the optimal dilutions be determined by titration experiments. 

For long storage, keep small aliquots of 1-2 μl at - 80°C (or at least - 20°C). For continuous use keep diluted solutions at 4°C (with 0.01% Na-azide). Avoid repeated freezing and thawing.

Porzig H., Li Z., Nicoll D.A., Philipson K.D. (1993) Mapping the cardiac sodium-calcium exchanger with monoclonal antibodies. Am. J. Physiol. 265:C78 -C756, 1993.