RM195 specifically reacts to Histone H4 mono- methylated at Arginine 3 (R3me1). No cross reactivity with unmodified Arg 3 (R3 ctrl), asymmetric dimethylated Arg 3 (R3me2a) or symmetric dimethylated Arg 3 (R3me2s), and citrullinated Arg 3 (R3Cit) in Histone H4.
Western Blot of (1) acid extracts of HeLa cells; (2) recombinant Histone H4. Using RM195 at 0.5 ug/mL, showed a band of Histone monomethylated at Arginine 3 in HeLa cells.
Immunocytochemical staining of HeLa cells treated with sodium butyrate, using anti-Monomethyl-Histone H4 (Arg3) Rabbit Monoclonal Antibody (clone RM195) (red). Actin filaments have been labeled with fluorescein phalloidin (green).
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