Anti-phospho-Rsk1 (Thr359/Ser363) Rabbit Monoclonal Antibody Data Sheet RM233
Specificity
RM233 reacts to human RSK1 only when phosphorylated at Thr359/Ser363.
Immunogen
A phospho-peptide corresponding to human phospho-Rsk1 (Thr359/Ser363)
Application
Immunohistochemistry (IHC)
Western Blot (WB)
Species Reactivity
Human
Conjugate
None
Intended Use
Research Use Only
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Properties
Form
Liquid
Storage Instructions
Store at -20.0°C
Stability
Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size
100 uL
Purity
Protein A affinity purified from an animal origin–free culture supernatant
Clone Type
Monoclonal
Isotype
Rabbit IgG
Usage
Immunohistochemistry (IHC): 1:200-1:500 dilution;
Western Blot (WB): 1:1000-1:2000 dilution.
Keywords
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Product Images
Western Blot of A431 cells treated (+) or nontreated (-) with EGF, using Anti-phospho-Rsk1 (Thr359/Ser363) RM233 at a 1:1000 dilution, showed a band of phospho- rylated Rsk1 only in EGF treated A431 cells .
Immunohistochemical staining of formalin fixed and paraffin embedded human cerebral cortex tissue sections using Anti-phospho-Rsk1 (Thr359/Ser363) RM233 at a 1:200 dilution.