RM201 specifically reacts to Histone H4 acetylated at Lysine 8 (K8ac). No cross reactivity with unmodified Lysine 8 (K8 ctrl), acetylated Lysine 5 (K5ac), Lysine 12 (K12ac), Lysine 16 (K16ac), Lysine 20 (K20ac), Lysine 31 (K31ac), or Lysine 91 (K91) in Histone H4.
Western Blot of (1) acid extracts of HeLa cells treated with sodium butyrate, (2) acid extracts of HeLa cells untreated, and (3) recombinant Histone H4. Using RM201 at 0.5 ug/mL, showed a band of Histone H4 acetylated at Lysine 8 in HeLa cells.
Immunocytochemical staining of HeLa cells treated with sodium butyrate, using anti-Acetyl-Histone H4 (Lys8) Rabbit Monoclonal Antibody (clone RM201) (red). Actin filaments have been labeled with fluorescein phalloidin (green).