Western blot of nonreduced(-) and reduced(+) rabbit IgG (20ng/lane), using 0.2ug/mL of RevMAb clone RMG01. This antibody reacts to nonreduced rabbit IgG (~150 kDa).
ELISA of IgGs from different species, shows RMG01 reacts to the Fab region of rabbit IgG; no cross reactivity with human IgG, rat IgG, or mouse IgG. The plate was coated with 50 ng/well of different IgG. 500 ng/mL, 200 ng/mL, or 50 ng/mL of RMG01 was used as the primary antibody. An alkaline phosphatase conjugated anti-goat IgG as the secondary antibody.
A titer ELISA of rabbit IgG. The plate was coated with different amounts of rabbit IgG. A serial dilution of RMG01 was used as the primary antibody. An alkaline phosphatase conjugated anti-goat IgG as the secondary antibody.
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