Human Monoclonal Antibody
This product is purified human IgE with kappa light chains produced in vitro from a monoclonal hybridoma. Original material is obtained from a healthy donor tested negative by US-FDA approved tests against HIV, HCV and Hepatitis B.Must be handled as potentially infectious as all human material.The purity of the human IgE is > 90%, tested by SDS-PAGE.As the IgE comes from a monoclonal cell line, there is no contamination of antibodies of other isotypes. The human IgE is purified by Protein L chromatography. The remaining contaminants are mainly components from Foetal Bovine Serum.
Non-immune
IgE/k
100 μl, 1mg/mL
ELISA This antibody is well suited as a standard in IgE quantifying assays due to its very low batch-to-batch variation.?
IHC This antibody can be used in immunochemistry
Cell Stimulation Asssay This antibody can be used in cellular immunology research.
Western Blot This antibody can be used in Western Blotting.
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See flyer for all major DIA HE1 major scientific references.
?Investigation of Aspergillus oryzae involvement in allergic bronchopulmonary aspergillosis (ABPA) 4. Yamaki K and Yoshino S (2011) Aspergillus oryzae Lectin Induces Anaphylactoid Oedema and Mast Cell Activation Through its Interaction With Fucose of Mast Cell–Bound Non-specific IgE. Scandinavian Journal of Immunology 74:445–453.
Used as a control for IgE in skin graft on mice 5. Fairley JA, Burnett CT, Fu CL, Larson DL, Fleming MG, Giudice GJ (2007) A pathogenic role for IgE in autoimmunity: Bullous pemphigoid IgE reproduces the early phase of lesion development in human skin grafted to nu/nu mice. J Invest Dermatol 127:2605-2611.
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